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KMID : 1149220200280020147
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Korean Journal of Oriental Medical Prescription
2020 Volume.28 No. 2 p.147 ~ p.155
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Anti-inflammatory Effects of Andrographis Herba MeOH Extract on LPS-induced Raw 264.7 Cells
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Jung Ji-Yun
Park Chung-A
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Abstract
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PURPOSE : Andrographis Herba is used as a traditional herbal medicine in the Asian countries for the treatment common cold, fever, diabetes, hypertension, hepatitis, skin infections, snake bite, and other diseases. In this study, we investigated the anti-inflammatory effect of MeOH extract of Andrographis Herba (AHME) on LPS-activated Raw 264.7 cells.
METHODS : Cell viability was determined by MTT assay. Nitric oxide (NO) production was determined by Griess reagent. Pro-inflammatory cytokines were detected by enzyme-linked immunosorbent assay. Expression levels of pro-inflammatory proteins were determined by Western blot analysis.
RESULTS : Production of NO in LPS activated Raw 264.7 cells, was significantly decreased by pre-treatment with 3-30 ㎍/mL of AHME. Production of pro-inflammatory mediators such as TNF-α and IL were significantly decreased by AHME 30 ㎍/mL pre-treatment. AHME significantly decreased p-IκB and NF-κB expression.
CONCLUSION : The results of this study indicate that AHME could inhibit the acute inflammatory response, via modulation of NF-κB activation.
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KEYWORD
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Andrographis Herba, inflammation, NF-κB
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